Name: Gabriella Xavier Maretto
Type: PhD thesis
Publication date: 09/03/2017

Namesort descending Role
Jose Geraldo Mill Co-advisor *
Suely Gomes de Figueiredo Advisor *

Examining board:

Namesort descending Role
Jose Geraldo Mill Co advisor *
Leonardo dos Santos Internal Examiner *
MARCIA HELENA BORGES External Examiner *
Rita Gomes Wanderley Pires External Examiner *
Suely Gomes de Figueiredo Advisor *

Summary: Post-infarction ventricular remodeling is a dynamic and complex process that occurs in response to myocardial damage. However, little is known about changes in the proteome of the cardiac chambers in the early phase remodeling after infarction and in the development of heart failure (IM-CI). The objective of this study was to evaluate protein changes in right ventricular (RV) and left ventricular (LV) remodeling of rats with signs of heart failure, 7 days after infarction, using proteomic analysis - comparative DIGE and mass spectrometry. The infarction was obtained by occlusion of the left coronary artery and heart failure confirmed by the analysis of the left ventricular enddiastolic pressure &#8805; 15 mmHg; increased lung mass / body mass ratio and presence of RV hypertrophy, with concomitant reduction in LV contractility and relaxation indexes. The quantitative analysis revealed differences in intensity / expression of 20 proteins in the RV and 11 in the LV of the infarcted animals with signs of heart failure (p <0.05), 14 of these proteins were identified by MALDI-TOF / TOF. Changes were detected in proteins related to energy metabolism (carbohydrates, lipids, amino acids and oxidative phosphorylation), heat shock proteins, muscle contraction and structural proteins and diverse functions. The changes included adaptive and malapable modulations and were different between the chambers. It was observed increased expression of desmin (intermediate filament protein), and decreased expression of creatine kinase and proteins involved in energy metabolism. These modulations were more expressive in the LV and may be associated with impairment in contractility and relaxation observed only in this chamber. On the other hand, a lower index of increase of the desmin and increase of the heat shock proteins (alpha-b-crystalline and HSP-71) were observed in the RV, considered adaptive modulations that can be associated to the preservation of the contractile machinery of the RV. The results showed that cardiac chambers present different protein remodeling 7 days after infarction, which may reflect the distinct impairment of the chambers, and suggest that the increase in desmin shows a positive correlation with the dysfunction ventricular. They also showed, for the first time, alterations in the RV proteome of animals with heart failure.

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