Name: JAMILA RODRIGUES BARBOZA
Publication date: 26/09/2016
Advisor:
Name |
Role |
|---|---|
| AGATA LAGES GAVA | Advisor * |
| SILVANA DOS SANTOS MEYRELLES | Co-advisor * |
Examining board:
| Name |
Role |
|---|---|
| AGATA LAGES GAVA | Advisor * |
| BRENO VALENTIM NOGUEIRA | External Examiner * |
| LEONARDO DOS SANTOS | Internal Examiner * |
| SILVANA DOS SANTOS MEYRELLES | Advisor * |
Summary: Acute kidney injury (AKI) is a multifactorial disease that affects thousands of people around the world. However, no specific therapy is available to treat acute ischemic kidney diseases, and new approaches are needed. This investigation aimed to evaluate the effects of kefir, a probiotic fermented milk, on renal function, oxidative stress and apoptosis in animals AKI. The induction of AKI was achieved in male Wistar rats through 45 minutes of renal ischemia and 24 hours of reperfusion. The animals were divided into five groups: control (Sham); Control kefir; ischemia/reperfusion (I/R) and I/R kefir (I/R/K). The kefir was administered daily by gavage at a dose of 0.3 mL/day per 100g body weight for 14 or 60 days before IR. In the groups did not received kefir were administered 0.3 mL/100g body weight of cow's milk per day Type C, pH adjusted to 4.3. After renal function was determined using inulin and para-aminohippurate (PAH) clearance, to quantify glomerular filtration rate, renal plasma flow, renal blood flow and renal vascular resistance. Renal cortex and medulla were removed to oxidative stress and evaluation of apoptosis by flow cytometry. Our results demonstrate that IR rats showed a decrease in the inulin and PAH clearances, and renal blood flow and an increase in renal vascular resistance when compared to Sham animals. Flow cytometry data showed significant increase in the production of superoxide anions and decreased nitric oxide production in animals with AKI when compared to the Sham group; these changes were ameliorated by treatment with kefir. It was also observed an increased apoptosis in the I/R group, and kefir significantly reduced the level of apoptosis. These results suggest that treatment with kefir can prevent renal injury induced by ischemia reperfusion, probably by reducing oxidative stress and apoptosis, indicating that kefir may be used as non-pharmacological adjuvant to slow the progression of renal complications. Keywords: ischemia reperfusion; acute kidney injury; kefir.
