Name: OSCAR MAURICIO SANTAMARIA NIÑO

Publication date: 17/01/2022
Advisor:

Name	Role
JONES BERNARDES GRACELI	Advisor *

Examining board:

Name	Role
JONES BERNARDES GRACELI	Advisor *
LEANDRO CEOTTO FREITAS LIMA	External Examiner *
ROGER LYRIO DOS SANTOS	Internal Examiner *

Summary: Obesity is associated with female reproductive complications, such as polycystic ovary syndrome (SOP), is linked with obesity and insulin resistance (IR). The exact mechanism of this relationship remains unclear. We identified a Few studies that used refined carbohydrate (HCD) to lead to obesity. This investigation assessed whether subchronic HCD exposure results in SOP-like features and other irregularities. We fed the Female with HCD for 14 days, a sessing metabolic outcome and reproductive tract morphophysiology. We further evaluated reproductive tract inflammation, oxidative stress (OS), and fibrosis. HCD rats displayed metabolic impairments, such as an increased body weight/adiposity, associated to a metabolic imbalance.
Moreover, we observed an improper functioning of the HCD reproductive tract. Specifically, irregular estrous cyclicity, high LH levels and abnormal ovarian morphology coupled with reduction in primordial and primary follicle numbers were observed, suggesting ovarian reserve depletion. Improper follicular development and a reduction in antral follicles, corpora lutea and granulosa layer area together with an increase in cystic follicles were apparent. Uterine atrophy and reduction in endometrial gland (GE) number were observed in HCD rats. In addition, we identified reproductive tract inflammation, OS, and fibrosis in HCD rats. Further, we observed strong positive correlations between body weight/adiposity and IR with estrous cycle length, cystic follicles, ovarian reserve, GE and other reproductive abnormalities. Thus, these data suggest that the subchronic HCD exposure led ta SOP-like features, impaired ovarian reserve, GE number, and other reproductive abnormalities in female rats.

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